Active Motif | PIXUL Multi-Sample Sonicator. The PIXUL Multi-Sample Sonicator is the first and only multi-sample sonicator that delivers extremely consistent shearing of up to 96 samples processed in parallel and is fast, simple, and inexpensive to operate. Each cooler is also sold separately. Sonicator (e.g. Active Motif | Sonication Products Active Motif's PIXUL and EpiShear sonication products are ideal for shearing chromatin, DNA, RNA, and protein for use in chromatin immunoprecipitation (ChIP), DNA methylation studies, next-generation sequencing, and proteomics assays. With a programmable sonicator like Active Motif's EpiShear Probe Sonicator (Catalog No. This system utilizes ultrasound transducers to create focused ultrasonic waveforms, transmitted through PIXUL Coupling Fluid, to a 96-well microplate (cat. 53139). Up to 12 unique sonication conditions can be run on a single 96-well plate, allowing simultaneous processing of multiple sample types and rapid optimization of conditions for difficult samples . EpiShear Probe Sonicator (version A6) Catalog Nos. Active Motif North America 1914 Palomar Oaks Way, Suite 150 Toll Free: 877 222 9543 Carlsbad, CA 92008, USA Direct: 760 431 1263 Fax: 760 431 1351 E-mail: tech_service@activemotif.com Active Motif Europe Active Motif's EpiShear sonication systems are designed for ease of use and ultimate control to ensure the highest degree of sample-to-sample reproducibility. This system utilizes ultrasound transducers to create focused ultrasonic waveforms, transmitted through They immediately saw the potential of PIXUL to help their customers with sample preparation issues, and they understand the power of PIXUL. Compatible with chromatin, DNA, RNA, and protein from purified samples, cells, or tissues. The PIXUL Multi-Sample Sonicator from Active Motif combines the benefits of the existing sonication technologies, while also eliminating the drawbacks, resulting in a powerful and disruptive new approach to sonication that is changing the way researchers are able to perform sample preparation for modern multi-omics studies. Active Motif's EpiShear sonication products are ideal for shearing chromatin for use in chromatin immunoprecipitation (ChIP). However, . Active Motif's EpiShear Multi-Sample Sonicator is a high-intensity cup horn sonicator that can shear up to eight vials (20 l - 1.2 ml of sample / vial) of chromatin or DNA simultaneously for use in chromatin IP (ChIP), DNA methylation studies and Next-Gen sequencing. Rwik Sen, Ph.D. Field Applications Scientist, Active Motif "Being able to load the material in a standard 96 well plate and not need a a lot of . They focus on scientific discovery, and they bend over backwards to help their customers. chromatin for ChIP assays, and we at Active Motif highly recommend it. Most sonicators are compatible with methanol-buffered formaldehyde. Inexpensive consumables keep sample processing costs low. 53051) with the EpiShear Cooled Sonication Platform (Catalog No. 53080)) Agarose gel electrophoresis apparatus Razor blades (for tissue preparations) Hand-held homogenizer for tissue preparations (e.g. Manufacturer: Active Motif 53071 Because polystyrene transfers sonic wave energy much more efficiently than polypropylene, Active Motif highly recommends that its Polystyrene Sonication Tubes be used with the Q8000R Sonicator. Shearing by physical sonication, as opposed to enzymatic methods, is . The PIXUL Multi-Sample Sonicator is shipped at room temperature and should be operated in an environment of 15C - 25C and maximum relative humidity of 85%. If you need assistance at any time, please call or send an e-mail to Active Motif Technical Service at one of the locations listed below. It is ideal for shearing chromatin or DNA from small or large sample sizes for use in chromatin immunoprecipitation (ChIP), DNA methylation studies and Next-Gen sequencing. The EpiShear Probe Sonicator is a microprocessor-based, ultrasonic processor that offers both programmable and manual operation. Active Motif's PIXUL Multi-Sample Sonicator is a high-throughput instrument for mechanical shearing of DNA, RNA, chromatin, or protein from purified samples, cells, or tissue . The cooler can keep the sample cold for up to 2 hours of sonication. Determine levels of H3.1-containing, circulating cell-free nucleosomes Active Motif's Nu.Q H3.1 Assay Kit is designed for the detection of levels of Histone H3.1-containing, circulating cell-free nucleosomes (cf-nucleosomes) in human serum in a high-throughput format. Active Motif's EpiShear Sonicator with a 1/8" probe (Catalog No. rch PIXUL 3 Histone H3.1, or the canonical form of Histone H3, is deposited during . Active Motif | EpiShear Probe Sonicator. Active Motif EpiShear Probe Sonicator Methanol-buffered formaldehyde Amplitude: 25% Pulse: 30 seconds ON, 30 seconds OFF Cycles: 20 27460500 28455377 Covaris Focused-Ultrasonicator They were designed to save you. Keypad programming and digital displays clearly display all parameters and options, making it easy to control sonicator functions, such as amplitude, pulse . Active Motif | 5,263 followers on LinkedIn. Up to 12 unique sonication conditions can be run on a single 96-well plate, allowing simultaneous processing of multiple sample types and rapid optimization of conditions for difficult samples. 53051), you can simply set the duration of the On and Off pulses, place the sample in the cooler, press Start, then walk away. Active Motif's Ready-to-ChIP Chromatin is another useful reagent that will save you time. Enabling Epigenetics Research | What is exciting about the area we work in Epigenomics is the systematic analysis of the global state of gene expression . PIXUL is compatible with simultaneous processing of 1-96 samples in parallel to avoid sample shearing bottlenecks in NGS applications. Use of polypropylene tubes in indirect sonication has been shown to reduce the extent to which the chromatin is sheared. These high-quality chromatin samples are available from a variety of different cell types (HeLa, Hep G2, K-562 & NIH/3T3 cells); they have been sheared by sonication and are ready for use in ChIP. Next-generation sequencing applications, such as ChIP-Seq, RNA-Seq, RIP-Seq, exome sequencing, and whole genome sequencing require precise sample fragmentation (200-600 bp) for compatibility with short read sequencing platforms. Biospec Products Tissue-Tearor) Active Motif's PIXUL Multi-Sample Sonicator is a high-throughput instrument for mechanical shearing of DNA, RNA, chromatin, or protein from purified samples, cells, or tissue. no. Active Motif is a company for researchers. Each unit includes the following components: PIXUL instrument Power cord Drain hose PIXUL Multi-Sample Sonicator Publications Read independent reviews on EpiShear Probe Sonicator from Active Motif on SelectScience 53051 (110V) & 53052 (230V) Active Motif North America 1914 Palomar Oaks Way, Suite 150 Carlsbad, California 92008, USA Toll free: 877 222 9543 Telephone: 760 431 1263 Fax: 760 431 1351 Active Motif Europe Avenue Reine Astrid, 92 B-1330 La Hulpe, Belgium UK Free Phone: 0800 169 31 47
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